Dako Pascal Manual de usuario descargar pdf (Pagina 13)

Pascal Handbook

Section 4

Operating Instructions

Set Up

1. Familiarize yourself with Pascal and its parts prior to operating.

2. Adjust the pan by matching the pan handles with the body handles.

Note If the outside of the pan is wet, Pascal will make a cracking noise. Any water in the

body may cause malfunctions.

3. Fill the pan with 500 mL of deionized water for performing the standard Target Retrieval

procedure.

4. Ensure the heat shield is in the center of the pan.

5. Place 1 to 4 Tissue-Tek

containers each filled with 250 mL of suitable Target Retrieval

Solution in the pan. Alternatively, use plastic Coplin jars. Fill each plastic Coplin jar with

50 mL of Target Retrieval Solution. Place slides intended for the Target Retrieval

procedure in the Tissue-Tek

containers or Coplin jars.

6. Place a dry Pascal Quality Strip on top of the containers.

7. Position the lid on the pan and secure in place by aligning the OPEN arrow etched on

the lid’s surface with one of the white dots on the pan handle. Grip the lid handle and

rotate clockwise so that the CLOSE arrow is aligned with the white dot on the pan handle.

8. Place the weight (petcock) on the vent nozzle located on the Pascal chamber lid.

9. Turn the red main power switch to the ON position (I).

Programming

Pascal Factory Settings

Push the Display Set button to recall the following factory setting parameters on a new

instrument. These settings can be changed for individual applications. Refer to Changing

Factory Setting within this section for notes on making changes for specific applications.

Pascal Factory Settings

Factory Settings Temperature Time

SP1 125 ºC 30 seconds

SP2 90 ºC 10 Seconds

SP Limit 10 ºC n/a

1 2 ... 8 9 10 11 12 13 14 15 16 17 18 ... 75 76

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Dako Pascal Manual de usuario Pagina 13