Dako Autostainer Manual de usuario

Dako Autostainer | English Handbook

Do not print slide labels while the Autostainer is running. Immunohistochemistry is a multi-step diagnostic process that requires specialized trainin

Correct Disposal of this Product (according to Directive 2002/96/EC on Waste Electrical and Electronic Equipment [WEEE] applicable in the European Un

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Section 2 System Overview The Autostainer System is an automated slide processing system compatible with currently available reagents for staining p

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Section 3 Software Overview Sign In Screen The Sign In screen appears when you double-click the Autostainer icon on the desktop. After your name and

Initialize The Initialize screen is used to establish and update default information for the system. This includes the institution information, Autost

2. Select the desired option for each field. Note: Static information, such as the institution name, can be entered into the field. This informati

Printer Selection To select the proper Seymour printer type on the Design Slide Label screen, scroll though the printer list using the keyboard arro

Add Rinse to Protocol Steps: When creating a new protocol template, a buffer rinse can be added automatically, following each step. Select the Add R

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Prime Pump The Prime Pump screen is used to flush the intake tubing with buffer or DI water without initiating an actual run. Frequently running fluid

Section 4 Entering Slide Information Slides can be programmed from the Programming Grid either through Slides on the menu bar for quick programming

Deleting Slides Deleting Slides With the Slides Function When using the Slides option on the menu bar to program a staining run, a slide can be del

Section 5 Designing a Protocol Protocol templates allow you to enter the series of steps required to stain a slide and save the protocol for future

Additional Protocol Elements Rinse Buffer A buffer rinse step consists of a buffer wash and air blow cycle and should be programmed between each proto

Note: If No Pre-rinse is selected at the Options screen, the first rinse buffer is not displayed (See Initializing, Section 3). 3. Build the new pr

3. Click the Use Template button. The Programming Grid appears with the selected protocol template displayed in the grid header. Using a Saved Protoco

Section 6 Programming a Staining Run The Autostainer can process a maximum of 48 microscope slides in a single staining run. Each slide can be stain

4. Select Yes and, if the program is new, a dialog box prompts “Save program on disk?” Otherwise, the Slide Layout Map screen appears. Select Back to

Adding Positive and Negative Control Reagents 1. Enter Slide Information and select a protocol template. A minimum of one slide must be programmed t

Dako Autostainer Handbook Document Number 0000620 Revision A July 2006 Autostainer Handbook iii

antibody does not have either a compatibility or negative control type defined, an error appears stating that there is no matching negative control re

Deleting an Auto Programming Item 1. Select Auto from the menu bar on the Programming Grid. 2. Select Setup from the Auto menu. The word Auto changes

Editing a Protocol Step The Autostainer software provides multiple methods for editing the reagents in a defined staining run from the Programming Gri

Reagent Labels Printing reagent labels from a programmed run: 1. Program or retrieve a previously saved run on the Programming Grid, and select the

Assigning Reagent Dispense Locations The non-frosted area of each slide is equally divided into three horizontal sections – or dispense locations. Ea

Section 7 Reagent Management Reagent lists are created and modified using the commands on the Edit Lists menu on the Programming Grid. Adding Detec

Adding Primary Antibodies A primary antibody can be added to the reagent list with a related pretreatment reagent. This facilitates automatic selecti

Compatibility Check A compatibility check is included to minimize programming errors resulting from assigning incompatible reagents (such as alkaline

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Section 8 Loading Reagents After programming is complete, the Autostainer calculates the time required for the staining run, the amount of wash buf

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5. Check that the reagent vials have no bubbles on top of the solution. Remove any bubbles. Missing Reagent Notice The Autostainer displays a promp

Section 9 Loading Slides After programming is complete and the reagents are loaded, you are ready to load the slides on the slide racks. Print the Sl

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Section 10 Starting a Staining Run Preparing the System for a Staining Run After the specimen-containing slides and reagent vials are loaded, perfo

6. Select the Protocol Template button and select a protocol template for the run. 7. Select the Use Template button. The Programming Grid appears wi

Section 11 Maintenance and Troubleshooting The Autostainer should be cleaned after 150 slides have been processed or after one week, whichever com

Problem Possible Cause Course of Action Buffer or DI Water does not flow out during a run or a prime. (Cont.) Tubing is disconnected at the inlet f

Problem Possible Cause Course of Action The probe and wash/blow head are not going to the correct locations. The arm has been knocked out of calibra

Problem Possible Cause Course of Action Slide number is not listed on label. Label is not centered correctly on label. Reprint label if necessary an

Problem Possible Cause Course of Action Words on your monitor screen are too dim. The brightness control is not set properly. Adjust the brightnes

Copyright © 2006 Dako, Inc. All rights reserved. This document may not be copied in whole or in part or reproduced in any other media without the ex

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Section 12 System Specifications Hardware Specifications Dako Autostainer Dimensions 1.02m W x 0.69m D x 0.61m H 40" W x 27" D x 24"

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Section 13 Standard Configuration Surface Ensure that the area for the instrument is a solid, level surface that can support the weight of the syst

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Table of Contents Warnings, Precautions and Limitations... 1 System Overvi

Negative Controls....... 21 Defining Specific Rinses ...

Section 1 Warnings, Precautions and Limitations Keep the cover closed during operation. The robotic arm will move unexpectedly during the operatio